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Article

Recognition of oriT for DNA processing at termination of a round of conjugal transfer.

Journal of molecular biology - 28 Jul 2000

Becker E C, Meyer R J

Abstract excerpt

Conjugal transfer of plasmid DNA is terminated when the transferred strand, linearized at the 38 base-pair origin of transfer (oriT), is recircularized. For the plasmid R1162, it is the protein MobA, covalently linked to the linear strand, that rejoins the ends by a reversible transesterification reaction. We have identified from those oligonucleotides with a partially degenerate oriT base sequence,...

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Source

Identifiers

PMID: 10903855
DOI: 10.1006/jmbi.2000.3902

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

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Recognition of oriT for DNA processing at termination of a round of conjugal transfer.PMID 10903855Read ↗

TraY DNA recognition of its two F factor binding sites.Semantic proximity Read ↗

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Specific DNA recognition by F Factor TraY involves beta-sheet residues.Semantic proximity Read ↗

Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site.Semantic proximity Read ↗

Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone.Semantic proximity Read ↗

F plasmid conjugative DNA transfer: the TraI helicase activity is essential for DNA strand transfer.Semantic proximity Read ↗

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.Semantic proximity Read ↗

TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.Semantic proximity Read ↗

Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEsSemantic proximity Read ↗

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.Semantic proximity Read ↗

Essential motifs of relaxase (TraI) and TraG proteins involved in conjugative transfer of plasmid RP4.Semantic proximity Read ↗

Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM.Semantic proximity Read ↗

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