Article
The complex of oriC DNA with the DnaA initiator protein.
Research in microbiology - 1 Jan 2000
Messer W, Egan B, Gille H, Holz A, Schaefer C, Woelker B
Abstract excerpt
We describe several experimental approaches relating to the early steps in the initiation of DNA replication at oriC. 1) A matrix is given which enables calculatation of the relative affinity of DnaA boxes for DnaA protein; 2) base changes within single Dna A boxes in oriC have little effect on oriC function; 3) mutations which change the distance between DnaA boxes inactivate oriC, but changes by one helical...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation