Article
Inter- and intramolecular determinants of the specificity of single-stranded DNA binding and cleavage by the F factor relaxase.
Structure (London, England : 1993) - 1 Oct 2005
Larkin Chris, Datta Saumen, Harley Matthew J, Anderson Brian J, Ebie Alexandra, Hargreaves Victoria, Schildbach Joel F
Abstract excerpt
The TraI protein of conjugative plasmid F factor binds and cleaves a single-stranded region of the plasmid prior to transfer to a recipient. TraI36, an N-terminal TraI fragment, binds ssDNA with a subnanomolar K(D) and remarkable sequence specificity. The structure of the TraI36 Y16F variant bound to ssDNA reveals specificity determinants, including a ssDNA intramolecular 3 base interaction and two pockets within...
Topics
- Bacterial Proteins
- Binding Sites
- Crystallography, X-Ray
- DNA, Single-Stranded
- F Factor
- Genetic Variation
- Hydrogen Bonding
- Magnesium
- Models, Molecular
- Nucleic Acid Conformation
- Protein Binding
- Protein Structure, Secondary
- Protein Structure, Tertiary