Article
Mutational and physical analysis of F plasmid traY protein binding to oriT.
Molecular microbiology - 1 Feb 1994
Luo Y, Gao Q, Deonier R C
Abstract excerpt
F plasmid traY protein binding to wild-type or deleted regions containing the TraY-binding site, sbyA, was studied in vitro. The principal DNA-protein complex was formed with DNA segments including the sbyA site defined by footprinting and (with lesser affinity) with truncated segments that retained the leftward two-thirds of sbyA. This located the major sequence determinants for TraY binding between bp 204 and...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA Primers
- DNA-Binding Proteins
- Escherichia coli Proteins
- F Factor
- Molecular Sequence Data
- Mutation
- Protein Binding
- Sequence Homology, Nucleic Acid