Article
TraY DNA recognition of its two F factor binding sites.
Journal of molecular biology - 23 Aug 2002
Lum Pamela L, Rodgers Michael E, Schildbach Joel F
Abstract excerpt
F factor TraY, a ribbon-helix-helix DNA-binding protein, performs two roles in bacterial conjugation. TraY binds the F origin of transfer (oriT) to promote nicking of plasmid DNA prior to conjugative transfer. TraY also binds the P(Y) promoter to up-regulate tra gene expression. The two plasmid regions bound by TraY share limited sequence identity, yet TraY binds them with similar affinities. TraY recognition of...
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