Article
Essential motifs of relaxase (TraI) and TraG proteins involved in conjugative transfer of plasmid RP4.
Journal of bacteriology - 1 Jul 1994
Balzer D, Pansegrau W, Lanka E
Abstract excerpt
Two essential transfer genes of the conjugative plasmid RP4 were altered by site-directed mutagenesis: traG of the primase operon and traI of the relaxase operon. To evaluate effects on the transfer phenotype of the point mutations, we have reconstituted the RP4 transfer system by fusion of the t...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Conjugation, Genetic
- DNA Helicases
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Operon
- Phenotype
- Point Mutation
- R Factors
- Replicon