Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM.

Abstract excerpt

The conjugative transfer of bacterial F plasmids relies on TraM, a plasmid-encoded protein that recognizes multiple DNA sites to recruit the plasmid to the conjugative pore. In spite of the high degree of amino acid sequence conservation between TraM proteins, many of these proteins have markedly different DNA binding specificities that ensure the selective recruitment of a plasmid to its cognate pore. Here we...

Topics

• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Binding Sites
• Crystallography, X-Ray
• DNA, Bacterial
• Electrophoretic Mobility Shift Assay
• Escherichia coli
• Escherichia coli Proteins
• F Factor