Article
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.
Nucleic acids research - 1 Aug 2011
Wong Joyce J W, Lu Jun, Edwards Ross A, Frost Laura S, Glover J N Mark
Abstract excerpt
The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The crystal structure of the...
Topics
- Alleles
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- DNA, Bacterial
- DNA-Binding Proteins
- F Factor
- Models, Molecular
- Molecular Sequence Data
- Protein Binding
- Protein Structure, Tertiary