Article
F plasmid conjugative DNA transfer: the TraI helicase activity is essential for DNA strand transfer.
The Journal of biological chemistry - 26 Jan 2001
Matson S W, Sampson J K, Byrd D R
Abstract excerpt
The product of the Escherichia coli F plasmid traI gene is required for DNA transfer via bacterial conjugation. This bifunctional protein catalyzes the unwinding of duplex DNA and is a sequence-specific DNA transesterase. The latter activity provides the site- and strand-specific nick required to initiate DNA transfer. To address the role of the TraI helicase activity in conjugative DNA transfer traI mutants were...
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