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Article

Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site.

The Journal of biological chemistry - 19 Mar 2010

Lucas María, González-Pérez Blanca, Cabezas Matilde, Moncalian Gabriel, Rivas Germán, de la Cruz Fernando

Abstract excerpt

TrwC, the relaxase of plasmid R388, catalyzes a series of concerted DNA cleavage and strand transfer reactions on a specific site (nic) of its origin of transfer (oriT). nic contains the cleavage site and an adjacent inverted repeat (IR(2)). Mutation analysis in the nic region indicated that reco...

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Identifiers

PMID: 20061574
DOI: 10.1074/jbc.M109.057539
PMCID: PMC2838313

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-20061574-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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