Article
Smg1 nonsense mutations do not abolish nonsense-mediated mRNA decay in Drosophila melanogaster.
Genetics - 1 Sept 2005
Chen Zhenzhong, Smith Katherine R, Batterham Philip, Robin Charles
Abstract excerpt
Smg1 is a key component of nonsense-mediated decay (NMD) in Caenorhabditis elegans and mammals. Here we report that two nonsense alleles of the ortholog of Smg1 do not affect NMD in Drosophila melanogaster.
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Codon, Nonsense
- Crosses, Genetic
- Drosophila Proteins
- Drosophila melanogaster
- Female
- Male
- Protein Serine-Threonine Kinases
- RNA Interference
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic