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sacB-5-Fluoroorotic acid-pyrE-based bidirectional selection for integration of unmarked alleles into the chromosome of Rhodobacter capsulatus.

Applied and environmental microbiology - 1 Jun 2005

Yano Takahiro, Sanders Carsten, Catalano John, Daldal Fevzi

Abstract excerpt

The gram-negative, purple nonsulfur, facultative photosynthetic bacterium Rhodobacter capsulatus is a widely used model organism and has well-developed molecular genetics. In particular, interposon mutagenesis using selectable gene cartridges is frequently employed for construction of a variety of chromosomal knockout mutants. However, as the gene cartridges are often derived from antibiotic resistance-conferring...

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Identifiers

PMID: 15932997
DOI: 10.1128/AEM.71.6.3014-3024.2005
PMCID: PMC1151845

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Source receipt: pubmed, retrieved 7 Sept 2026.

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