Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus.

Extremophiles : life under extreme conditions - 1 Jul 2009

Deng Ling, Zhu Haojun, Chen Zhengjun, Liang Yun Xiang, She Qunxin

Abstract excerpt

Sulfolobus islandicus is being used as a model for studying archaeal biology, geo-biology and evolution. However, no genetic system is available for this organism. To produce an S. islandicus mutant suitable for genetic analyses, we screened for colonies with a spontaneous pyrEF mutation. One mutant was obtained containing only 233 bp of the original pyrE sequence in the mutant allele and it was used as a host to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Archaea
Share to beta-Galactosidase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Diploidy

Share to DNA, Archaeal

Share to Genetic Vectors

Share to Oligonucleotides

Share to Recombination, Genetic

Share to Sulfolobus

Source

Identifiers

PMID: 19513584
DOI: 10.1007/s00792-009-0254-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus.PMID 19513584Read ↗

Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus.Semantic proximity Read ↗

Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi.Semantic proximity Read ↗

Expanding the genetic toolbox for the obligate human pathogen <i>Streptococcus pyogenes</i>Semantic proximity Read ↗

Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenesSemantic proximity Read ↗

Endogenous mutagenesis in recombinant sulfolobus plasmids.Semantic proximity Read ↗

sacB-5-Fluoroorotic acid-pyrE-based bidirectional selection for integration of unmarked alleles into the chromosome of Rhodobacter capsulatus.Semantic proximity Read ↗

pING family of conjugative plasmids from the extremely thermophilic archaeon Sulfolobus islandicus: insights into recombination and conjugation in Crenarchaeota.Semantic proximity Read ↗

Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.Semantic proximity Read ↗

Generation of readily transformable Bacillus licheniformis mutants.Semantic proximity Read ↗

Highly Effective Markerless Genetic Manipulation of Streptococcus suis Using a Mutated PheS-Based Counterselectable MarkerSemantic proximity Read ↗

Degeneration of industrial bacteria caused by genetic instability.Semantic proximity Read ↗

High-Frequency Targeted Mutagenesis in Pseudomonas stutzeri Using a Vector-Free Allele-Exchange Protocol.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.