Article
Generation of Marked and Markerless Mutants in Model Cyanobacterial Species.
Journal of visualized experiments : JoVE - 29 May 2016
Lea-Smith David J, Vasudevan Ravendran, Howe Christopher J
Abstract excerpt
Cyanobacteria are ecologically important organisms and potential platforms for production of biofuels and useful industrial products. Genetic manipulation of cyanobacteria, especially model organisms such as Synechocystis sp. PCC6803 and Synechococcus sp. PCC7002, is a key tool for both basic and applied research. Generation of unmarked mutants, whereby chromosomal alterations are introduced into a strain via...
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