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Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatum.

Microbiology spectrum - 7 Jan 2025

Zhou Peng, G C Bibek, Hu Bo, Wu Chenggang

Abstract excerpt

Fusobacterium nucleatum, prevalent in the oral cavity, is significantly linked to overall human health. Our molecular comprehension of its role in oral biofilm formation and its interactions with the host under various pathological circumstances has seen considerable advancements in recent years, primarily due to the development of various genetic tools for DNA manipulation in this bacterium. Of these,...

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PMID: 39611826
DOI: 10.1128/spectrum.02066-24
PMCID: PMC11705814

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