Article
Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences.
Journal of bacteriology - 1 Jun 1990
Cai Y P, Wolk C P
Abstract excerpt
Use of the sacB gene (J. L. Ried and A. Collmer, Gene 57:239-246, 1987) provides a simple, effective, positive selection for double recombinants in Anabaena sp. strain PCC 7120, a filamentous cyanobacterium. This gene, which encodes the secretory levansucrase of Bacillus subtilis, was inserted into the vector portion of a suicide plasmid bearing a mutant version of a chromosomal gene. Cells of colonies in which...
Topics
- Cyanobacteria
- DNA Transposable Elements
- Genes, Lethal
- Mutation
- Nitrogenase
- Plasmids
- Recombination, Genetic