Article
Colorimetric approach to high-throughput mutation analysis.
BioTechniques - 1 Apr 2005
Benoit Nicole E, Goldenberg David, Deng Shirley X, Rosenbaum Eli, Cohen Yoram, Califano Joseph A, Shackelford William H, Wang Xiao B, Sidransky David
Abstract excerpt
High-throughput genomic mutation screening for primary tumors has characteristically been expensive, labor-intensive, and inadequate to detect low levels of mutation in a background of wild-type signal. We present a new, combined PCR and colorimetric approach that is inexpensive, simple, and can detect the presence of 1% mutation in a background of wild-type. We compared manual dideoxy sequencing of p53 for eight...
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