Article
A simple p53 functional assay for screening cell lines, blood, and tumors.
Proceedings of the National Academy of Sciences of the United States of America - 25 Apr 1995
Flaman J M, Frebourg T, Moreau V, Charbonnier F, Martin C, Chappuis P, Sappino A P, Limacher I M, Bron L, Benhattar J
Abstract excerpt
Mutations in the p53 gene are implicated in the pathogenesis of half of all human tumors. We have developed a simple functional assay for p53 mutation in which human p53 expressed in Saccharomyces cerevisiae activates transcription of the ADE2 gene. Consequently, yeast colonies containing wild-ty...
Topics
- Adolescent
- Adult
- Aged
- Base Sequence
- Carboxy-Lyases
- Cell Line
- Cloning, Molecular
- Codon
- DNA Primers
- Female
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genes, p53
- Humans
- Lymphocytes
- Male