Article
A simple p53 functional assay for screening cell lines, blood, and tumors.
Proceedings of the National Academy of Sciences of the United States of America - 25 Apr 1995
Flaman J M, Frebourg T, Moreau V, Charbonnier F, Martin C, Chappuis P, Sappino A P, Limacher I M, Bron L, Benhattar J
Abstract excerpt
Mutations in the p53 gene are implicated in the pathogenesis of half of all human tumors. We have developed a simple functional assay for p53 mutation in which human p53 expressed in Saccharomyces cerevisiae activates transcription of the ADE2 gene. Consequently, yeast colonies containing wild-type p53 are white and colonies containing mutant p53 are red. Since this assay tests the critical biological function of...
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