Article
Single-molecule detection of cancer mutations using a novel PCR-LDR-qPCR assay.
Human mutation - 1 May 2020
Ruiz Cristian, Huang Jianmin, Giardina Sarah F, Feinberg Philip B, Mirza Aashiq H, Bacolod Manny D, Soper Steven A, Barany Francis
Abstract excerpt
Detection of low-abundance mutations in cell-free DNA is being used to identify early cancer and early cancer recurrence. Here, we report a new PCR-LDR-qPCR assay capable of detecting point mutations at a single-molecule resolution in the presence of an excess of wild-type DNA. Major features of the assay include selective amplification and detection of mutant DNA employing multiple nested primer-binding regions...
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