Article
Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity and altered specificity.
Biochemistry - 16 Jul 1991
Vermersch P S, Lemon D D, Tesmer J J, Quiocho F A
Abstract excerpt
In addition to hydrogen bonds, van der Waals forces contribute to the affinity of protein-carbohydrate interactions. Nonpolar van der Waals contacts in the complexes of the L-arabinose-binding protein (ABP) with monosaccharides have been studied by means of site-directed mutagenesis, equilibrium...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Carbohydrate Conformation
- Carrier Proteins
- Escherichia coli Proteins
- Kinetics
- Leucine
- Methionine
- Molecular Sequence Data
- Monosaccharides
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- X-Ray Diffraction