Article
Characterization of recombinant rat cathepsin B and nonglycosylated mutants expressed in yeast. New insights into the pH dependence of cathepsin B-catalyzed hydrolyses.
The Journal of biological chemistry - 5 Mar 1992
Hasnain S, Hirama T, Tam A, Mort J S
Abstract excerpt
The cysteine proteinase rat cathepsin B was expressed in yeast in an active form and was found to be heterogeneously glycosylated at the consensus sequence for N-linked oligosaccharide substitution. Purified enzyme fractions containing the highest levels of glycosylation were shown to have reduce...
Topics
- Animals
- Catalysis
- Cathepsin B
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Glycosylation
- Hydrogen-Ion Concentration
- Hydrolysis
- Kinetics
- Liver
- Mutation
- Rats
- Recombinant Proteins
- Saccharomyces cerevisiae
- Substrate Specificity