Article
Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
Biotechnology and bioengineering - 1 Dec 2012
Hirakawa Hidehiko, Ishikawa Suguru, Nagamune Teruyuki
Abstract excerpt
The catalytic activity of Staphylococcus aureus sortase A (SaSrtA) is dependent on Ca(2+), because binding of Ca(2+) to Glu residues distal to the active site stabilizes the substrate binding site. To obtain Ca(2+)-independent SaSrtA, we substituted two Glu residues in the Ca(2+)-binding pocket (Glu(105) and Glu(108)). Although single mutations decreased SaSrtA activity, mutations of both Glu(105) and Glu(108)...
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