Article
Low accumulation of L90M in protease from subtype F HIV-1 with resistance to protease inhibitors is caused by the L89M polymorphism.
The Journal of infectious diseases - 1 Jun 2005
Calazans Alexandre, Brindeiro Rodrigo, Brindeiro Patricia, Verli Hugo, Arruda Monica B, Gonzalez Luis Manuel Felipe, Guimaraes Jorge A, Diaz Ricardo Sobhie, Antunes Octavio A C, Tanuri Amilcar
Abstract excerpt
BACKGROUND: This work evaluates the role of subtype F human immunodeficiency virus type 1 (HIV-1) protease (PR) substitutions L89M and L90M in viral replication and resistance to PR inhibitors (PIs). METHODS: Subtype B and F PR genes were subjected to site-directed mutagenesis, to create and reverse the methionine at positions 89 and 90. Viruses were re-created in cell culture, and their replicative capacity was...
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