Polymerase chain reaction/sequencing analysis of ras mutations in paraffin-embedded tissues as compared with 3T3 transfection and polymerase chain reaction/sequencing of frozen tumor deoxyribonucleic acids.

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Article

Polymerase chain reaction/sequencing analysis of ras mutations in paraffin-embedded tissues as compared with 3T3 transfection and polymerase chain reaction/sequencing of frozen tumor deoxyribonucleic acids.

Laboratory investigation; a journal of technical methods and pathology - 1 Apr 1992

Goodrow T L, Prahalada S R, Storer R D, Manam S V, Leander K R, Kraynak A R, van Zwieten M J, Nichols W W, Bradley M O

Abstract excerpt

The efficiency of detection of H- and K-ras mutations in 27 CD-1 mouse liver tumors by direct sequencing of polymerase chain reaction (PCR)-amplified DNA isolated from formalin-fixed and paraffin-embedded tissues was compared with that after assay by both NIH 3T3 transfection (followed by sequencing of amplified transformant DNA) and direct sequencing of PCR-amplified DNA isolated from frozen tumors. Some tumor...

Topics

3T3 Cells
Animals
Base Sequence
DNA, Neoplasm
Genes, ras
Histocytochemistry
Liver Neoplasms, Experimental
Male
Mice
Microtomy

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Hybridization

Paraffin Embedding

Polymerase Chain Reaction

Tissue Preservation

Transfection

Source

Open in PubMed

Identifiers

PMID: 1583889

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1583889-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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