Article
A sensitive restriction fragment length polymorphism method to detect CAA-->AAA mutations at codon 61 of Ha-ras.
Molecular carcinogenesis - 1 Jan 1993
Shinder G A, Manam S, Nichols W W
Abstract excerpt
A rapid and sensitive assay was developed to detect CAA-->AAA mutations at codon 61 of Ha-ras. The region surrounding codon 61 was amplified by the polymerase chain reaction (PCR) using one primer containing a mismatch at the second position of codon 60. Using this primer creates an Msel restrict...
Topics
- 3T3 Cells
- Alleles
- Animals
- Base Sequence
- Codon
- DNA, Neoplasm
- Deoxyribonucleases, Type II Site-Specific
- Genes, ras
- Liver Neoplasms
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Transfection