Article
ACB-PCR measurement of spontaneous and furan-induced H-ras codon 61 CAA to CTA and CAA to AAA mutation in B6C3F1 mouse liver.
Environmental and molecular mutagenesis - 1 Oct 2013
Banda Malathi, Recio Leslie, Parsons Barbara L
Abstract excerpt
Furan is a rodent liver carcinogen, but the mode of action for furan hepatocarcinogenicity is unclear. H-ras codon 61 mutations have been detected in spontaneous liver tumors of B6C3F1 mice, and the fraction of liver tumors carrying H-ras codon 61 CAA to AAA mutation increased in furan-treated mice. Allele-specific competitive blocker PCR (ACB-PCR) has been used previously to quantify early, carcinogen-induced...
Topics
- Animals
- Codon
- Female
- Furans
- Gene Amplification
- Genes, ras
- Liver Neoplasms
- Mice
- Mutagens
- Mutation
- Polymerase Chain Reaction