Article
Analysis of the rat ribosomal DNA promoter: characterization of linker-scanning mutants and of the binding of UBF.
Nucleic acids research - 11 Apr 1992
Xie W, O'Mahony D J, Smith S D, Lowe D, Rothblum L I
Abstract excerpt
To investigate the mechanism of transcription of the rat ribosomal DNA (rDNA) promoter, a series of 23 linker-scanning mutants were constructed and assayed in transfected CHO cells and with cell-free extracts. With minor variation, the results of the in vitro and in vivo assays paralleled one ano...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cell Extracts
- Cricetinae
- DNA
- DNA Mutational Analysis
- DNA, Ribosomal
- DNA-Binding Proteins
- Molecular Sequence Data
- Mutation
- Pol1 Transcription Initiation Complex Proteins
- Promoter Regions, Genetic
- RNA Polymerase I
- Rats
- Transcription Factors
- Transfection