Article
In vivo analyses of upstream promoter sequence elements in the 5 S rRNA gene from Saccharomyces cerevisiae.
Journal of molecular biology - 27 Jun 1997
Lee Y, Wong W M, Guyer D, Erkine A M, Nazar R N
Abstract excerpt
Upstream promoter elements of the Saccharomyces cerevisiae 5 S rRNA gene have been characterized by genomic DNase I "footprinting" and by in vivo mutational analyses using base substitutions and deletions. A high copy shuttle-vector was used to efficiently express the mutant 5 S rRNA genes in viv...
Topics
- Base Sequence
- Conserved Sequence
- DNA Footprinting
- DNA Mutational Analysis
- DNA, Ribosomal
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Mutation
- Promoter Regions, Genetic
- RNA, Ribosomal, 5S
- Saccharomyces cerevisiae