Article
The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro.
Molecular and cellular biology - 1 Sept 1990
Henderson S L, Sollner-Webb B
Abstract excerpt
Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be...
Topics
- Animals
- Cell Line
- Chromosome Deletion
- DNA Polymerase I
- DNA, Ribosomal
- Mice
- Mutation
- Promoter Regions, Genetic
- Templates, Genetic
- Terminator Regions, Genetic
- Transfection