Article
The yeast RNA polymerase I promoter: ribosomal DNA sequences involved in transcription initiation and complex formation in vitro.
Nucleic acids research - 11 Oct 1991
Kulkens T, Riggs D L, Heck J D, Planta R J, Nomura M
Abstract excerpt
Using an in vitro transcription system for Saccharomyces cerevisiae RNA polymerase I, we have analyzed Pol I promoter deletion mutants and mapped the boundaries of the promoter between positions -155 and +27. The 5'-boundary of the minimal core promoter capable of transcription initiation, however, was found to lie between -38 and -26. The 3'-deletion extending to -2 and -5 still allowed some transcription,...
Topics
- Base Sequence
- DNA, Fungal
- DNA, Ribosomal
- Escherichia coli
- Genes, Fungal
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA Polymerase I
- Saccharomyces cerevisiae