Article
Activity of promoter mutants of the yeast ribosomal RNA gene with and without the enhancer.
Yeast (Chichester, England) - 1 Oct 1991
Butlin M, Quincey R
Abstract excerpt
The promoter and enhancer of the rRNA gene of Saccharomyces cerevisiae have been studied using a nuclease S1 protection assay to detect transcripts of an rRNA minigene in transformed yeast. Analysis of 5' deletion mutants showed that DNA between -163 bp and -155 bp was important for promoter activity and that some DNA between -155 bp and -145 bp was essential. The importance of DNA far upstream from the...
Read the complete abstract on PubMedTopics
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- DNA, Ribosomal
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Fungal Proteins
