Article
Resistance to Pseudomonas toxin A: a sensitive marker to screen for mutagenic substances using V79 cells.
Mutagenesis - 1 Mar 1992
Suter W, Negro L, Barrera I, Schneider B
Abstract excerpt
The goal of this study was the development of a mutagenicity assay in V79 cells using Pseudomonas toxin A (PTA) as a selective agent and to compare it with the V79/hypoxanthineguaninephosphoribosyl transferase (HGPT) assay. PTA inhibits protein synthesis by covalently modifying elongation factor 2 (EF-2). Mutations in the EF-2 gene, in genes responsible for the modification of EF-2, and also in genes for the...
Topics
- ADP Ribose Transferases
- Aflatoxin B1
- Animals
- Bacterial Toxins
- Biomarkers
- Cell Line
- Cricetinae
- Drug Resistance
- Exotoxins
- Hypoxanthine Phosphoribosyltransferase
- Methyl Methanesulfonate