Article
Efficiency assessment of the gene trap approach.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 1998
Voss A K, Thomas T, Gruss P
Abstract excerpt
The trapping of genes in murine embryonic stem (ES) cells offers three features in one experimental approach: 1) analysis of the expression patterns of unknown genes by using a simple staining method, 2) rapid cloning of unknown genes, and 3) generation of mutant mouse lines. We performed a gene...
Topics
- Animals
- Base Sequence
- Chimera
- Cloning, Molecular
- DNA Primers
- DNA, Recombinant
- Embryonic and Fetal Development
- Female
- Gene Expression
- Genetic Techniques
- Homozygote
- In Vitro Techniques
- Mice
- Mice, Mutant Strains
- Mutagenesis, Insertional
- Mutation
- Pregnancy
- Random Amplified Polymorphic DNA Technique