The folding of an enzyme. IV. Structure of an intermediate in the refolding of barnase analysed by a protein engineering procedure.

doi:10.1016/0022-2836(92)90564-z

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Article

The folding of an enzyme. IV. Structure of an intermediate in the refolding of barnase analysed by a protein engineering procedure.

Journal of molecular biology - 5 Apr 1992

Matouschek A, Serrano L, Fersht A R

Abstract excerpt

The pathway of refolding of barnase has been analysed by the protein engineering method using phi plots. The description comprises a folding intermediate, a major transition state (the unfolding transition state) and the fully folded structure. Over 40 mutations have been analysed in the differen...

Topics

Amino Acid Sequence
Bacillus
Bacterial Proteins
Kinetics
Mutation
Protein Conformation
Protein Engineering
Ribonucleases
Structure-Activity Relationship

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