Article
The folding of an enzyme. III. Structure of the transition state for unfolding of barnase analysed by a protein engineering procedure.
Journal of molecular biology - 5 Apr 1992
Serrano L, Matouschek A, Fersht A R
Abstract excerpt
The structure of the first significant transition state on the unfolding pathway of barnase has been analysed in detail by protein engineering methods. Over 50 mutations placed strategically over the whole protein have been used as probes to report on the local structure in the transition state. Several different probes for many regions of the protein give consistent results as do multiple probes at the same...
Topics
- Amino Acid Sequence
- Bacillus
- Bacterial Proteins
- Kinetics
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Engineering
- Ribonucleases
- Structure-Activity Relationship