Article
Confirmation of the hierarchical folding of RNase H: a protein engineering study.
Nature structural biology - 1 Sept 1999
Raschke T M, Kho J, Marqusee S
Abstract excerpt
The kinetic intermediate of RNase H is structured in a core region of the protein. To probe the role of this intermediate in the folding of RNase H, the folding kinetics of mutant proteins with altered native state stabilities were investigated. Mutations within the folding core destabilize the kinetic intermediate and slow refolding in a manner consistent with an obligatory intermediate model. Mutations outside...
Topics
- Amino Acid Substitution
- Circular Dichroism
- Enzyme Stability
- Kinetics
- Models, Molecular
- Mutation
- Protein Denaturation
- Protein Engineering
- Protein Folding
- Protein Structure, Secondary
- Ribonuclease H