Article
Transient folding intermediates characterized by protein engineering.
Nature - 2 Aug 1990
Matouschek A, Kellis J T, Serrano L, Bycroft M, Fersht A R
Abstract excerpt
Kinetic experiments on engineered mutants of barnase detect an intermediate on the folding pathway and allow the mapping of the tertiary interactions of the side chains and their energetics. Many of the interactions present in the final folded state tend to be either fully formed or not formed at all in the intermediate or subsequent transition state for folding, but the hydrophobic core becomes progressively...
Topics
- Bacillus
- Bacterial Proteins
- Chemical Phenomena
- Chemistry, Physical
- Kinetics
- Magnetic Resonance Spectroscopy
- Mutation
- Protein Conformation
- Protein Engineering
- Ribonucleases
- Thermodynamics