Article
Refolding of barnase mutants and pro-barnase in the presence and absence of GroEL.
The EMBO journal - 1 Nov 1993
Gray T E, Eder J, Bycroft M, Day A G, Fersht A R
Abstract excerpt
Three mutants of barnase and a pro-barnase variant, which have a variety of different physical properties but the same overall protein structure, were analysed for their folding in the presence of the molecular chaperone GroEL. Mutants were chosen on the basis that changes in their refolding rate constants in solution are not correlated with the changes in their stability. All barnase variants fold considerably...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Chaperonin 60
- Heat-Shock Proteins
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Folding
- Protein Precursors