Article
One face of a transmembrane helix is crucial in mechanosensitive channel gating.
Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 1998
Ou X, Blount P, Hoffman R J, Kung C
Abstract excerpt
MscL is a mechanosensitive channel in bacteria that responds directly to membrane tension by opening a large conductance pore. To determine functionally important residues within this molecule, we have randomly mutagenized mscL, expressed the genes in living bacteria, and screened for gain-of-fun...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Cell Division
- Cell Membrane Permeability
- Electric Conductivity
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Ion Channel Gating
- Ion Channels
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis
- Osmolar Concentration
- Patch-Clamp Techniques
- Phenotype
- Potassium
- Protein Structure, Secondary