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Article

Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner.

The Journal of molecular diagnostics : JMD - 1 Feb 2005

Akbari Mansour, Hansen Marianne Doré, Halgunset Jostein, Skorpen Frank, Krokan Hans E

Abstract excerpt

Paraffin-embedded tissue is an important source of material for molecular pathology and genetic investigations. We used DNA isolated from microdissected formalin-fixed, paraffin-embedded gastric tumors for mutation analysis of a region of the human gene for uracil-DNA glycosylase (UNG), encoding the UNG catalytic domain, and detected apparent base substitutions which, after further investigation, proved to be...

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Identifiers

PMID: 15681472
DOI: 10.1016/s1525-1578(10)60006-2
PMCID: PMC1867510

Genes

UNG

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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