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Sequence Artifacts in DNA from Formalin-Fixed Tissues: Causes and Strategies for Minimization

24 Nov 2014

Abstract excerpt

BACKGROUND: Precision medicine is dependent on identifying actionable mutations in tumors. Accurate detection of mutations is often problematic in formalin-fixed paraffin-embedded (FFPE) tissues. DNA extracted from formalin-fixed tissues is fragmented and also contains DNA lesions that are the sources of sequence artifacts. Sequence artifacts can be difficult to distinguish from true mutations, especially in the...

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PMID: 25421801
DOI: 10.1373/clinchem.2014.223040

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