Article
Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Dec 2016
Arbeithuber Barbara, Makova Kateryna D, Tiemann-Boege Irene
Abstract excerpt
The need in cancer research or evolutionary biology to detect rare mutations or variants present at very low frequencies (<10-5) poses an increasing demand on lowering the detection limits of available methods. Here we demonstrated that amplifiable DNA lesions introduce important error sources in ultrasensitive technologies such as single molecule PCR (smPCR) applications (e.g. droplet-digital PCR), or...
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