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Article

PCR artifacts in LOH and MSI analysis of microdissected tumor cells.

Human pathology - 1 Nov 2000

Sieben N L, ter Haar N T, Cornelisse C J, Fleuren G J, Cleton-Jansen A M

Abstract excerpt

Polymerase chain reaction (PCR) analysis to study loss of heterozygosity (LOH) and microsatellite instability (MSI) in tumors is widely used. Microdissection techniques are applied to obtain tumor-specific tissue cells. By microdissection, however, the amount of template DNA extracted may vary considerably and interfere with optimal PCR amplification. To circumvent LOH and MSI misinterpretations due to low DNA...

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PMID: 11112218

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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