Article
Reducing Artifactual EGFR T790M Mutations in DNA from Formalin-Fixed Paraffin-Embedded Tissue by Use of Thymine-DNA Glycosylase.
Clinical chemistry - 1 Sept 2017
Do Hongdo, Molania Ramyar, Mitchell Paul L, Vaiskunaite Rita, Murdoch John D, Dobrovic Alexander
Abstract excerpt
BACKGROUND: False-positive EGFR T790M mutations have been reported in formalin-fixed lung tumors, but the cause of the false positives has not been identified. The T790M mutation results from a C>T change at the cytosine of a CpG dinucleotide. The presence or absence of methylation at this cytosine has different consequences following deamination, resulting in a thymine or uracil, respectively, both of which...
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