Article

Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applications

2017-09-13

Abstract excerpt

Expanded CAG/CTG repeats underlie the aetiology of 14 neurological and neuromuscular disorders. The size of the repeat tract determines in large part the severity of these disorders with longer tracts causing more severe phenotypes. Expanded CAG/CTG repeats are also unstable in somatic tissues, which is thought to modify disease progression. Routine molecular biology applications involving these repeats, including...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a3524500-00d8-58b5-8a69-044b6ff2290f
DOI: 10.1101/187872

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applicationsDOI 10.1101/187872Read

Short Tandem Repeats of Human Genome Are Intrinsically Unstable in Cultured Cells <i>in vivo</i>Semantic proximity Read

FFPE DNA shows two major error profiles derived from deamination of cytosine and methylcytosine that can be mitigated using distinct repair strategiesSemantic proximity Read

Identification of a CCG-enriched expanded allele in DM1 patients using Amplification-free long-read sequencingSemantic proximity Read

When repetita no-longer iuvant: somatic instability of the CAG triplet in Huntington’s diseaseSemantic proximity Read

Identification of a CCG-Enriched Expanded Allele in Patients with Myotonic Dystrophy Type 1 Using Amplification-Free Long-Read Sequencing.Semantic proximity Read

Insights into the causes and consequences of DNA repeat expansions from 700,000 biobank participantsSemantic proximity Read

Systematic microsatellite repeat expansion cloning and validation.Semantic proximity Read

Detailed analysis of HTT repeat elements in human blood using targeted amplification-free long-read sequencing.Semantic proximity Read

Detection of slipped-DNAs at the trinucleotide repeats of the myotonic dystrophy type I disease locus in patient tissues.Semantic proximity Read

Approaches to Sequence the HTT CAG Repeat Expansion and Quantify Repeat Length VariationSemantic proximity Read

Simple procedure for automatic detection of unstable alleles in the myotonic dystrophy and Huntington's disease loci.Semantic proximity Read

Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner.Semantic proximity Read