Article
Cytosine deamination is a major cause of baseline noise in next-generation sequencing.
Molecular diagnosis & therapy - 1 Oct 2014
Chen Guoli, Mosier Stacy, Gocke Christopher D, Lin Ming-Tseh, Eshleman James R
Abstract excerpt
BACKGROUND AND OBJECTIVES: As next-generation sequencing (NGS) becomes a major sequencing platform in clinical diagnostic laboratories, it is critical to identify artifacts that constitute baseline noise and may interfere with detection of low-level gene mutations. This is especially critical for applications requiring ultrasensitive detection, such as molecular relapse of solid tumors and early detection of...
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