Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Awareness of mutational artefacts in suboptimal DNA samples: possible risk for therapeutic choices.

Expert review of molecular diagnostics - 1 May 2018

Mariani Sara, Bertero Luca, Coppola Vittoria, Saracco Giorgio, Arezzo Alberto, Francia Di Celle Paola, Metovic Jasna, Marchiò Caterina, Cassoni Paola

Abstract excerpt

BACKGROUND: Technical biases due to PCR artefacts could represent an insidious obstacle for mutational analysis and precision medicine. METHODS: The authors report a retrospective analysis by fast COLD-PCR and sequencing of 31 suboptimal tumor DNA samples obtained from FFPE tissues and liquid biopsies. RESULTS: In FFPE tumor tissues and plasma liquid biopsies of patients with lung and colorectal adenocarcinoma,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Artifacts
Share to Biomarkers, Tumor

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Neoplasm

Share to High-Throughput Nucleotide Sequencing

Share to Liquid Biopsy

Share to Neoplasms

Share to Precision Medicine

Share to ras Proteins

Source

Identifiers

PMID: 29676606
DOI: 10.1080/14737159.2018.1468254

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Awareness of mutational artefacts in suboptimal DNA samples: possible risk for therapeutic choices.PMID 29676606Read ↗

Sequence Artifacts in DNA from Formalin-Fixed Tissues: Causes and Strategies for MinimizationSemantic proximity Read ↗

Dramatic reduction of sequence artefacts from DNA isolated from formalin-fixed cancer biopsies by treatment with uracil- DNA glycosylase.Semantic proximity Read ↗

Extreme assay sensitivity in molecular diagnostics further unveils intratumour heterogeneity in metastatic colorectal cancer as well as artifactual low-frequency mutations in the KRAS gene.Semantic proximity Read ↗

Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications.Semantic proximity Read ↗

Sequence artefacts in a prospective series of formalin-fixed tumours tested for mutations in hotspot regions by massively parallel sequencing.Semantic proximity Read ↗

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.Semantic proximity Read ↗

Cytosine deamination is a major cause of baseline noise in next-generation sequencing.Semantic proximity Read ↗

COLD-PCR: Applications and Advantages.Semantic proximity Read ↗

Formalin fixation increases deamination mutation signature but should not lead to false positive mutations in clinical practice.Semantic proximity Read ↗

Reducing sequence artifacts in amplicon-based massively parallel sequencing of formalin-fixed paraffin-embedded DNA by enzymatic depletion of uracil-containing templates.Semantic proximity Read ↗

Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner.Semantic proximity Read ↗

Targeted KRAS mutation assessment on patient tumor histologic material in real time diagnostics.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.