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Article

Structure-function analysis of human transforming growth factor-alpha by site-directed mutagenesis.

The Biochemical journal - 1 Apr 1992

Feild J A, Reid R H, Rieman D J, Kline T P, Sathe G, Greig R G, Anzano M A

Abstract excerpt

Site-directed mutants of transforming growth factor-alpha (TGF-alpha) were expressed in an Escherichia coli outer membrane protein A (ompA) expression/secretion vector under the transcriptional control of the lambda PL promoter. TGF-alpha mutant proteins were isolated from cell pellets using alkaline extraction with 0.1 M-Tris (pH 10.5). The levels of protein expression of 23 TGF-alpha mutants were comparable...

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Identifiers

PMID: 1567383
DOI: 10.1042/bj2830091
PMCID: PMC1130998

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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