Article
Mutational analysis of structure--activity relationships in human tumor necrosis factor-alpha.
Protein engineering - 1 Aug 1990
Yamagishi J, Kawashima H, Matsuo N, Ohue M, Yamayoshi M, Fukui T, Kotani H, Furuta R, Nakano K, Yamada M
Abstract excerpt
To determine the region of human tumor necrosis factor-alpha (TNF-alpha), essential for cytotoxic activity against mouse L-M cells, single amino-acid-substituted TNF-alpha mutant proteins (muteins) were produced in Escherichia coli by protein engineering techniques. An expression plasmid for TNF-...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cell Survival
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes
- Humans
- Hydrogen-Ion Concentration
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Engineering
- Sequence Homology, Nucleic Acid
- Structure-Activity Relationship
- Tumor Necrosis Factor-alpha