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Article

Production and characterization of an analog of acidic fibroblast growth factor with enhanced stability and biological activity.

Protein engineering - 1 Jul 1993

Arakawa T, Horan T P, Narhi L O, Rees D C, Schiffer S G, Holst P L, Prestrelski S J, Tsai L B, Fox G M

Abstract excerpt

We have used recombinant DNA methods to produce two forms of bovine acidic fibroblast growth factor (aFGF), one with alanine substituted for the cysteine at position 47 and the other with the Ala47 change plus the substitution of glycine for the naturally occurring histidine at position 93. Both forms were expressed at high levels in Escherichia coli and purified to near homogeneity by solubilization of the...

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PMID: 7692436
DOI: 10.1093/protein/6.5.541

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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