Article
Production and characterization of an analog of acidic fibroblast growth factor with enhanced stability and biological activity.
Protein engineering - 1 Jul 1993
Arakawa T, Horan T P, Narhi L O, Rees D C, Schiffer S G, Holst P L, Prestrelski S J, Tsai L B, Fox G M
Abstract excerpt
We have used recombinant DNA methods to produce two forms of bovine acidic fibroblast growth factor (aFGF), one with alanine substituted for the cysteine at position 47 and the other with the Ala47 change plus the substitution of glycine for the naturally occurring histidine at position 93. Both...
Topics
- 3T3 Cells
- Alanine
- Amino Acid Sequence
- Animals
- Base Sequence
- Biological Assay
- Cell Division
- Circular Dichroism
- Cysteine
- Escherichia coli
- Fibroblast Growth Factor 1
- Glycine
- Histidine
- Hot Temperature
- Mice
- Molecular Sequence Data
- Mutation
- Protein Denaturation